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Karina G's picture

Lab 9

Kalyn Schofield

Karina Granadeno

Bio 103, Lab 9, Mendel's Garden Revisited
Fly Lab
Fly Traits:
 
Fly Experiment
Remember: Try and find some traits that yield unexpected results in a monohybrid cross, as well as some that yield unexpected results in a dihybrid cross.
TRUE BREEDING – No variation from initial parents to young with continued breeding.
F1 Generation (+) is all wild type trait. (3 to 1 ratio of wild VS Ebony/ degree of freedom)
F2 Ebony (E) type trait.
Original parents (P)
Variation with one element for both parents.
Each gene in two different states with parents giving one copy from each parent. Every individual has two versions of a gene.
Phenotype –Appearance
Genotype – Actual genes present
++ = Homozygous (SAME)
+E = Heterozygous (DIFFERENT)
RESULTS:
1.)    Monohybrid Cross:
2.)    Dihybrid Cross:
 
Dihybrid Cross: ++, VGVG, EE, VGE
Experiment: 1
ORIGINAL PARENTS Female Fly: Wild Type (+)
ORIGINAL PARENTS Male Fly: Spineless Type (SS)
F1
Female: + 494
Male: + 482
F2
Female: +369
Female: SS 123
Male: +364
Male: SS 111
RESULTS
Chi-Squared Test Statistics: 0.9614
Degrees of Freedom: 3
Level of Significance: 0.8106
Ratio : 3:1
 
Experiment: 2
ORIGINAL PARENT: Female Fly: Wild Type (+)
ORIGINAL PARENT: Male Black: Wild Type(+); PR (PURPLE) Eyes; BL (Black Body)
F1
Female: + 508
Male: +484
F2
Female + 376; PR 12, BL 9; PRBL 97
Male + 360; PR 9; BL 8; PRBL 111
 
Conclusions: We have discovered that for the F1 generation using original parents that carry a wild type and a single variance such as spineless create the 3 to 1 ratio in their offspring. But when the original parent (single) has two variances such as eye color and wing type you receive the 2 to 2 ratio. Our problem with the third experiment was when both parents had two different variances it still generated the 2 to 2 ratio. This third experiment suggests that the trait variances are significant and will dictate the offspring ratio. So it’s not that Mendel’s trait formula of 3 to 1 and 9 to 3 are wrong it’s that it only applies to specific traits that are possibly dominant. You would need to test all flies in order to come to a definite conclusion.
We also ran other experiments and concluded that some crossings are not possible.

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