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jfl's picture

Ethyl Alcohol

Submitted by jfl (guest) on Fri, 04/24/2009 - 8:26pm.

The procedure for this DNA extraction of the strawberries said that instead of using cold ethyl alcohol one ". . .can use 1X volume of room temperature isopropanol, rubbing alcohol, as a substitute." I want to understand both technically and in simple terms why pure ethyl alcohol must be chilled, but rubbing alcohol needs to remain at room temp. The alcohol normally floats to the top of the DNA does it not? Please explain. Also, do you know who actually did the strawberry experiement? Please forward this to them, or reply their email.

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